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KMID : 0613820120220081009
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Journal of Life Science
2012 Volume.22 No. 8 p.1009 ~ p.1017
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Analysis of the orf 282 Gene and Its Function in Rhodobacter sphaeroide 2.4.1
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Son Myung-Hwa
Lee Sang-Joon
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Abstract
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The orf282 gene of Rhodobacter sphaeroides is located between the ccoNOQP operon encoding cbb₃ terminal oxidase and the fnrL gene encoding an anaerobic activator, FnrL. Its function remains unknown. In an attempt to reveal the function of the orf282 gene, we disrupted the gene by deleting a portion of the orf282 gene and constructed an orf282-knockout mutant. Two FnrL binding sites were found to be located upstream of orf282, and it was demonstrated that orf282 is positively regulated by FnrL. The orf282 gene is not involved in the regulation of spectral complex formation. The cbb₃ oxidase activity detected in the orf282 mutant was comparable to that in the wild-type sample, indicating that the orf282 gene is not involved in the regulation of the ccoNOQP operon and the biosynthesis of the cbb3 cytochrome c oxidase. The elevated promoter activity of the nifH and nifA genes, which are the structural genes of nitrogenase and its regulator, respectively, in the orf282 mutant, suggests that the orf282 gene product acts as a negative effector for nifH and nifA expression.
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KEYWORD
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Rhodobacter sphaeroides, nitrogenase, the orf282 gene, the nifH gene, the nifA gene
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